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EZ-Mutation™

  • Overview
  • Tech Specs
  • Benefits
  • Support Docs

Product Overview

The EZ-Mutation™ site-directed DNA mutagenesis kit employs the simplest and fastest technology available to introduce a wide variety of mutations into small plasmids. It supports plasmids up to 12 kbp without the need for sub-cloning or the availability of unique restriction sites. This technology requires the amplification of the full target plasmid; therefore, we recommend it for plasmids smaller than 6.0 kbp. For medium and large plasmids we recommend using our GenEdit™ kit (Cat# 201321 to 201323).

Tech Specs

  • Catalog #: 201304, 201305, 201306 for 6, 16, and 24 reactions respectively.
  • Kit size: 8, 16, or 24 reactions.
  • Maximum Target plasmid size: Up to 12 kbp, recommended for 6.0 kbp.
  • Maximum insertion mutation size: Up to 100 bp in any combination.
  • Maximum deletion mutation size: Up to 10,000 bp.
  • Unique restriction sites requirements: Restriction sites are not required.
  • Polymerase: proprietary FBT HotStart High Fidelity DNA polymerase.

Storage Info

  • Storage temperature of E.Coli cells: -80C
  • Storage temperature for kit reagents: -20C in a non frost-free freezer.
  • Shelf life: 1 year from date of receipt.

Product Benefits

  • Fastest mutagenesis method: It involves a single inverse PCR reaction followed by transformation to obtain mutant clones within 2-3 days.
  • Convenience: all key reagents are premixed and distributed in single reaction tubes, ready for reaction.
  • Cloning vectors are not required: Most mutagenesis projects involving large genes or plasmids require cloning a section of the original plasmid into smaller cloning vectors. EZ-Mutation™ does not require any cloning vectors.
  • No special E. Coli strains are needed: This gives you the flexibility to use any transformation cells of your choice. FBT Super Competent cells are shipped with the kit.
  • Cloning, sub-cloning or restriction sites are not required: Your mutations will be introduced to your plasmid by a set of mutagenic primers.
  • Targeted DNA Polymerization: DNA polymerization is limited only to the mutagenesis target site, not the full gene or plasmid under investigation, removing the need for full sequence verification.
  • Simple protocol: Easy to follow instructions for both the novice and seasoned researchers. It takes only a few minutes to set up each reaction steps.
  • Unlimited free technical support

Support Docs

EZ Mutation User’s Guide v1.32