The GenEdit™ site-directed DNA mutagenesis kit employs the most versatile and powerful technology available today. It is designed to facilitate direct mutagenesis of plasmids up to 50,000 base pairs without sub-cloning. This technology requires amplification of only the mutagenesis target region, not the full plasmid.
The procedure involves two modular units. The first unit is a series of biochemical reactions that can be completed in one day. The second unit involves cloning the final PCR product from the first unit into your target plasmid using conventional cloning into restriction sites. Under optimal conditions, both modular units can be finished in…
The EZ-Mutation™ site-directed DNA mutagenesis kit employs the simplest and fastest technology available to introduce a wide variety of mutations into small plasmids. It supports plasmids up to 12 kbp without the need for sub-cloning or the availability of unique restriction sites. This technology requires the amplification of the full target plasmid; therefore, we recommend it for plasmids smaller than 6.0 kbp. For medium and large plasmids we recommend using our GenEdit™ kit (Cat# 201321 to 201323).